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Table 5 Statistical comparison between the results obtained by the proposed HPLC &HPTLC methods and the reported method for analysis of TER in its pure powdered form

From: Validated chromatographic methods for determination of teriflunomide and investigation of its intrinsic stability

Parameters

Proposed Methods

Reported method

HPLC

HPTLC

HPLC(19)*

HPTLC(18)**

Mean Recovery

100.02%±0.39

99.50%±0.74

99.81%±0.47

99.11%±0.80

n

9

9

9

9

SD

0.39

0.74

0.47

0.80

Variance

0.156

0.553

0.225

0.654

Student t-test

1.08(2.12)

1.09(2.12)

  

F-Test

1.44(2.44)

1.18(2.44)

  
  1. * RP-HPLC using C18, end-capped (250 × 4.6) mm, 5 μm, at 250 nm. The mobile phase A was buffer pH 3.0; while, the mobile phase B was acetonitrile (ACN) (100%) in gradient mode flow rate 1 ml/min
  2. **HPTLC-densitometric method was done on aluminium plates coated with silica gel 60 F254 utilizing toluene: ethyl acetate: glacial acetic acid (7.5:2: 0.5 v/v/v) as the mobile phase at UV 284 nm
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BMC Chemistry

ISSN: 2661-801X

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